Research Article
miR193b Promotes Apoptosis of Gastric Cancer Cells via Directly Mediating the Akt Pathway
Figure 4
P-Akt is an upstream regulator of miR193b. (a) The expression of miR-193b was negatively correlated with the phosphorylation of Akt in gastric cancer cells. (b) qRT-PCR measurements of miR193b expression in cells transfected with Myr-Akt1 or treated with Akt inhibitor (LY294002). (c, d) Western blot measurements of KRAS protein after treatment with IGF-1 or Myr-Akt1. (e, f) miR193b reduced the inhibitory effect of IGF-1/Myr-Akt1 on apoptosis of SGC-7901 cells. (g, h) Western blot measurement of cleaved-caspase3, bax, and bcl-2 in cells overexpressing miR193b after IGF-1/Myr-Akt1 treatment. and were calculated using Student’s -test.
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