Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis

Figure 3

Protein and mRNA levels of OPN, integrin ανβ3, and HAS in OA chondrocytes. (a, b) OA chondrocytes showed increased protein levels of OPN, integrin ανβ3, and HAS. Immunoblots also showed binding between OPN and integrin ανβ3. (c) The mRNA levels of OPN, integrin ανβ3, and HAS were also higher in the OA chondrocytes. (d, e) We generated three plasmids bearing the siRNA against OPN. Although we observed similar rates of OPN depletion, we selected siRNA-1 for the subsequent experiments. , , .

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