The Development and Application of a Base Editor in Biomedicine

Figure 2

Schematic of prime editor works in target DNA. (a) The PE-pegRNA complex binds the target DNA and nicks the PAM-containing strand. The target strand’s 3 end is hybridized with the primer-binding site, then primers reverse transcription of new DNA containing the desire edit using the RT template of the pegRNA. (b) Flap equilibration in prime editing. Left panel represents the failure of hybridization. The 3 DNA flap containing edited information is excised, resulting in target sites unchanged. Right panel shows the hybridization to DNA unmodified strand, and DNA repair process introduces mutation to the second DNA strand.

(a)

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