The Development and Application of a Base Editor in Biomedicine

Figure 3

Engineering dCas13b-ADAR fusions for RNA editing. Schematic of RNA editing by dCas13b-ADAR_DD fusion protein which naturally deaminates adenosines to inosines in the target RNA. The crRNA specifies the target site by hybridization firstly, then creates a double strand RNA (dsRNA) structure and recruits the dCas13b-ADAR_DD fusion protein to deaminize. Besides, a mismatched cytidine in the crRNA is opposite the target adenosine which can enhance the conversion of target A to I.