The Development and Application of a Base Editor in Biomedicine

Table 1

Overview of different base editor variants.

First author	Publication years	Species	Category of base editor	Cas protein	Deaminase	Editing windows	PAM
Yuan Zong [49]	2017	Rice, wheat, and maize	PBE	nSpCas9	rAPOBEC1	3-9	NGG
Puping Liang [50]	2017	Human	YEE-BE3	nSpCas9	rAPOBEC1-YEE	5-6	NGG
Y Bill Kim [24]	2017	Human	SaBE3	nSaCas9	rAPOBEC1	3-12 for SaBE3	NNGRRT for SaBE3
			SaKKH-BE3	nSaCas9-KKH		3-15 for SaKKH-BE3	NNNRRT for SaKKH-BE3
			VQR-BE3	nSpCas9-VQR		4-11 for VQR-BE3 and	NGAN for VQR-BE3
			EQR-BE3	nSpCas9-VRER		EQR-BE3	NGAG for EQR-BE3
			RER-BE3			3-10 for VRER-BE3	NGCG for VRER-BE3
Pranam Chatterjee [51]	2018	Human	ScCas9-ABE7.10	ScCas9(n)	TadA-TadA	4-9	NNGN
Noah Jakimo [52]	2018	Human	Spy-mac nCas9-BE3	nSp-macCas9	rAPOBEC1	4-7	NAAN
Hiroshi Nishmasu [53]	2018	Human	Target-AID-NG	SpCas9(n)	PmCDA1	2-8	NG
Xiao Wang [31]	2018	Human	hA3A-BE3	SpCas9(n)	hAPOBEC3A	2-13	NGG
Xiaosa Li [29]	2018	Human	dCpf1-BE	dLbCpf1	rAPOBEC1	8-13 for dCpf1-BE and dCpf1-eBE	TTTV
			dCpf1-BE-YE		rAPOBEC1-YE	10-12 for dCpf1-BE-YE and dCpf1-eBE-YE
			dCpf1-eBE
			dCpf1-eBE-YE
Johnny H. Hu [28]	2018	Human	xCas9-BE3	nxCas9	APOBEC1	4-8	NG, GAA and GAT
			xCas9(3.7)-ABE		TadA-TadA
Xinjian Li [54]	2020	Human	CjABE	nCjCas9	TadA-TadA	9	GGGGACC
Shannon M. Miller [55]	2020	Human	CBE-NRRH	nSpCas9-NRRH	APOBEC1 for CBE	4-8 for CBE	NRRH
			CBE-NRCH	nSpCas9-NRCH	TadA-TadA for ABE	4-7 for ABE
			CBE-NRTH	nSpCas9-NRTH
			ABE-NRRH
			ABE-NRCH
			ABE-NRTH
Michelle F. Richter [32]	2020	Human	SpABE8e	nSpCas9	TadA-8e	4-8 for SpABE8e	NGG for SpABE8e
			SaABE8e	nSaCas9		3-14 for SaABE8e	NNGRRT for SaABE8e
			LbABE8e	dLbCas12a		8-14 for LbABE8e and enAsABE8e	TTTV for LbABE8e and
			EnAsABE8e	denAs-Cas12a			enAsABE8e
Xiang Lin [56]	2020	Human	SaCas9n-KKH-miniABEmax	nSaCas9-KKH	TadA	N.R.	NNNRRT
Ziying Hu [57]	2020	Human	SauriBE4max	nSauriCas9	APOBEC1 for CBE	4-13 for CBE	NNGG
			SauriABEmax		TadA-TadA for ABE	6-14 for ABE
Russell T. Walton [58]	2020	Human	SpG-BE4max	nSpCas9	rAPOBEC1 for CBE	3-9 for CBE	NGNN for SpG
			SpG-ABEmax		TadA-TadA for ABE	5-7 for ABE	NRN for SpRY (R is A or G)
			SpRY-CBE
			SpRY-ABE