Significance of EGFR/HER2 Expression and PIK3CA Mutations in Giant Cell Tumour of Bone Development

Figure 2

Expression level of HER2, EGFR, and RANKL. (a) Expression of HER2 in GCTB using immunohistochemistry and appropriate antibodies. Moderate level (++), picture labelled in light green, and strong level (+++), picture labelled in dark green, respectively. HER2 is localized in the cytoplasm of giant cells. The arrowheads indicate positive staining (×200). (b) Expression of EGFR in GCTB using immunohistochemistry and appropriate antibodies. Weak expression level (+), picture labelled in yellow; moderate level (++), picture labelled in light green; and strong level (+++), picture labelled in dark green, respectively. EGFR is localized in both types of cells, giant cells and some mononuclear cells. The arrowheads indicate positive staining (×200). (c) Expression of RANKL in GCTB using immunohistochemistry and appropriate antibodies. Weak expression level (+), picture labelled in yellow; moderate level (++), picture labelled in light green; and strong level (+++), picture labelled in dark green, respectively. RANKL is localized in the cytoplasm of the stromal and giant cells. The arrowheads indicate positive staining (×200).

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