Research Article
Alterations of the Predominant Fecal Microbiota and Disruption of the Gut Mucosal Barrier in Patients with Early-Stage Colorectal Cancer
Table 2
Bacterial-specific primer sets for detection of fecal microbiota by qPCR.
| PCR specificity | Primer | Sequence (5→3) | Annealing temperature (°C) | Amplicon size (bp) |
| Total bacteria | Uni331F | TCCTACGGGAGGCAGCAGT | 58 | 466 | Uni797R | GGACTACCAGGGTATCTATCCTGTT | | | Bacteroides-Prevotella group | Bac303F | GAAGGTCCCCCACATTG | 56 | 418 | Bac708R | CAATCGGAGTTCTTCG | | | Lactobacillus spp. | Lac-F | AGCAGTAGGGAATCTTCCA | 58 | 341 | Lac-R | CACCGCTACACATGGAG | | | Bifidobacterium spp. | Bifid-F | CTCCTGGAAACGGGTGG | 55 | 550 | Bifid-R | GGTGTTCTTCCCGATATCTACA | | | Clostridium cluster I | CG1-F | TACCHRAGGAGGAAGCCAC | 63 | 700 | CG1-R | GTTCTTCCTAATCTCTACGCAT | | | Clostridium cluster XI | CG2-F | ACGCTACTTGAGGAGGA | 58 | 141 | CG2-R | GAGCCGTAGCCTTTCACT | | | Clostridium cluster XIVab | CG3-F | GAWGAAGTATYTCGGTATGT | 52 | 152 | CG3-R | CTACGCWCCCTTTACAC | | | Faecalibacterium prausnitzii | Fpra-F | GATGGCCTCGCGTCCGATTAG | 58 | 199 | Fpra-R | CCGAAGACCTTCTTCCTCC | | | Fusobacterium nucleatum | Fn-F | CAACCATTACTTTAACTCTACCATGTTCA | 60 | 112 | Fn-R | GTTGACTTTACAGAAGGAGATTATGTAAAAATC | | | Enterobacteriaceae | Eco-F | CATTGACGTTACCCGCGAGAAGAAGC | 63 | 195 | Eco-R | CTCTACGAGCTCAAGCTTGC | | | Enterococcus spp. | ENco-F | CCCTTATTGTTAGTTGCCATCATT | 61 | 144 | ENco-R | ACTCGTTGTACTTCCCATTGT | | |
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