Alterations of the Predominant Fecal Microbiota and Disruption of the Gut Mucosal Barrier in Patients with Early-Stage Colorectal Cancer

Table 2

Bacterial-specific primer sets for detection of fecal microbiota by qPCR.

PCR specificity	Primer	Sequence (5→3)	Annealing temperature (°C)	Amplicon size (bp)
Total bacteria	Uni331F	TCCTACGGGAGGCAGCAGT	58	466
	Uni797R	GGACTACCAGGGTATCTATCCTGTT
Bacteroides-Prevotella group	Bac303F	GAAGGTCCCCCACATTG	56	418
	Bac708R	CAATCGGAGTTCTTCG
Lactobacillus spp.	Lac-F	AGCAGTAGGGAATCTTCCA	58	341
	Lac-R	CACCGCTACACATGGAG
Bifidobacterium spp.	Bifid-F	CTCCTGGAAACGGGTGG	55	550
	Bifid-R	GGTGTTCTTCCCGATATCTACA
Clostridium cluster I	CG1-F	TACCHRAGGAGGAAGCCAC	63	700
	CG1-R	GTTCTTCCTAATCTCTACGCAT
Clostridium cluster XI	CG2-F	ACGCTACTTGAGGAGGA	58	141
	CG2-R	GAGCCGTAGCCTTTCACT
Clostridium cluster XIVab	CG3-F	GAWGAAGTATYTCGGTATGT	52	152
	CG3-R	CTACGCWCCCTTTACAC
Faecalibacterium prausnitzii	Fpra-F	GATGGCCTCGCGTCCGATTAG	58	199
	Fpra-R	CCGAAGACCTTCTTCCTCC
Fusobacterium nucleatum	Fn-F	CAACCATTACTTTAACTCTACCATGTTCA	60	112
	Fn-R	GTTGACTTTACAGAAGGAGATTATGTAAAAATC
Enterobacteriaceae	Eco-F	CATTGACGTTACCCGCGAGAAGAAGC	63	195
	Eco-R	CTCTACGAGCTCAAGCTTGC
Enterococcus spp.	ENco-F	CCCTTATTGTTAGTTGCCATCATT	61	144
	ENco-R	ACTCGTTGTACTTCCCATTGT