Structure-Activity Relationship (SAR) and in vitro Predictions of Mutagenic and Carcinogenic Activities of Ixodicidal Ethyl-Carbamates
Table 5
Mutagenicity of carbamate LQM 996 towards Salmonella typhimurium TA98, TA100, TA1535, TA1537 and TA102 strain with or without S9 metabolic activation.
No. of his revertant colonies/plate (mean ± SD)
Concentration (mg/mL)
TA98
TA100
TA1535
TA1537
TA102
-S9
+S9
-S9
+S9
-S9
+S9
-S9
+S9
-S9
+S9
0 (-)
18.5 ± 3.2
27.7 ± 2.5
104.1 ± 10.8
120 ± 9
9.1 ± 3.9
16.5 ± 1.9
9.1 ± 3
14.6 ± 3
319.5 ± 75.7
330 ± 66
0.1
20.3 ± 4.1
28.3 ± 6.1
94.6 ± 27.7
92.6 ± 5.5
8.3 ± 3.2
7.6 ± 4.6
7.3 ± 3.2
8.3 ± 1.1
395.3 ± 6.1
473 ± 17.5
0.2
20 ± 5
30.3 ± 4.0
96 ± 3
101.3 ± 12.3
4 ± 1.7
10.6 ± 3.5
4.6 ± 2
8.3 ± 2.5
251.6 ± 49.8
419.6 ± 31.6
0.4
17 ± 4
22.6 ± 8
83.6 ± 17.6
95 ± 4.3
2.6 ± 1.5
11.6 ± 1.5
1.6 ± 2.8
5.3 ± 3
241.3 ± 49.1
367.3 ± 58.2
0.5
18.3 ± 4
30.3 ± 1.1
96.6 ± 12.5
81.6 ± 16.6
2.6 ± 2.3
4.6 ± 3.2
0
0
194.3 ± 43
335.6 ± 18
1
13 ± 1.7
21.3 ± 3
34.6 ± 26.2
0
0
6.6 ± 2.8
0
0
150 ± 16.7
185.3 ± 29.2
Limit value:
37
55.4
208.2
240
18.2
33
18.2
29.2
639
660
Positive controls:
2-NF
2017 ± 460
Sodium azide
1666 ± 99
731 ± 77.2
Picrolonic acid
48.33 ± 25.1
4-NQO
1573 ± 61
2-AA
1831 ± 163
2277 ± 146
765 ± 45
441 ± 99
2050 ± 93
S9 = Microsomal fraction of liver of rats treated with phenobarbital and beta-naphthoflavone enriched with NADP and glucose-6-P; 2-NF =2-nitrofluorene; 4-NQO =4-nitroquinolido N-oxide; 2-AA =2-aminoanthracene.
