Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression
Establishment of the rat septic shock model. (a) Mean arterial pressure of rats from the control and sepsis groups. (b) Pathological changes in rat heart tissues from the control and sepsis groups after LPS injection for 3, 6, or 12 hours by H&E staining. The following results were obtained from rat heart tissues 12 hours post-LPS treatment. (c) Apoptosis was detected using the TUNEL assay. Green represents the cardiomyocytes, red represents the TUNEL positive cells, and blue represents the cell nucleus. (d) The expression of caspase3 mRNA was analyzed by qPCR. (e) The expression of cleaved caspase3 protein was assayed by western blot. (f) The function of target genes of differentially expressed miRNAs from sepsis and control groups was determined by GO enrichment. (g) qPCR validation of selected miRNAs in the rat heart tissues from the control and sepsis groups, . Data are presented as , repeated for three times. compared to the control.