The Impact of Bioactive Surfaces in the Early Stages of Osseointegration: An In Vitro Comparative Study Evaluating the HAnano® and SLActive® Super Hydrophilic Surfaces

Figure 2

HAnano® triggers intracellular signaling through integrin activation in 3 and 24 hours of attachment. (a) Scheme of the signaling pathway downstream upon integrin activation. Transcriptional profile determination of integrin (b) β1, (c) Fak, (d) Src, (e) Cdk2, (f) Cdk4, and (g) Cdk6 genes after 3 h and 24 h of osteoblast adhesion by qPCR technology. The relative gene expression levels were determined using the cycle threshold (Ct) method and showed in a graphical format with normalized values as a function of the control assigned value 1. The results represented as of three independent experiments. 3 h: when compared to Ctrl and when compared to SLActive®; 24 h: , , , and when compared to Ctrl and and when compared to SLActive®.

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