Dissecting the Interaction Deficiency of a Cartilaginous Fish Digestive Lipase with Pancreatic Colipase: Biochemical and Structural Insights

Figure 6

Lipase-colipase interaction. (a) Ribbon representation of SmDL and HPL interaction with colipase. The HPL structure is in complex with colipase (PDB code 1LPB). HPL and SmDL structures are well superposed. Residues involved in the lipase-colipase interaction are shown as sticks. (b) Zoom in from (a) showing the interaction interface between superimposed lipases (HPL and SmDL) and colipase. Residues conserved between the two lipases are shown only once. Residue Y404 in HPL and the corresponding residue in SmDL (N404) are both shown, and each of them is in interaction with colipase residue R65, shown in red. The hydrogen bond between R65 of the colipase and Y404 of HPL is shown as a dotted line.