Apatinib Inhibits the Invasion and Metastasis of Liver Cancer Cells by Downregulating MMP-Related Proteins via Regulation of the NF-κB Signaling Pathway

Figure 3

Real-time PCR and Western blot analysis of expression levels of MMP family genes in Hep3B and HepG2 liver cells. (a) Real-time PCR analysis of mRNA levels of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-10, MMP-11, and MMP-16. Compared with the control group, the expression levels of the mRNAs of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-10, MMP-11, and MMP-16 in the apatinib-treated group were significantly decreased (). (b) Western blot was used to screen the level of MMP expression in the HCC cell lines, showing that apatinib reduced the expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-10, MMP-11, and MMP-16. GAPDH was used as the loading control. MMP: matrix metalloproteinase.

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