Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots

<div>Characterization of recombinant sdAbE2s by indirect ELISA and Western blot. Binding activity of sdAbE2 with grE2 protein was analyzed by indirect ELISA and western blot. (a) A dilutions series of each sdAbE2s were used for detection grE2 protein by indirect ELISA with HRP conjugated anti-HIS-antibody as the secondary antibody. (b) The analysis of sdAbE2s against grE2 binding capacity by western blot. CSFV immune serum and preimmune serum were used as positive (lane C+) and negative control (lane C-). (c) The analysis of sdAbE2s against E2 of CSFV in ST cells by western blot. <i>β</i>-actin was used as an internal reference protein. Error bars represent ±SD of three independent measurements.</div>

BioMed Research International

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Figure 3

Figure 3: Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots 

Figure 3 | Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots 