Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots

<div>Capture of IMNBs to CSF virus and grE2 protein. Lysates of CSFV (C-strain) infected ST cells and grE2 protein were incubated with IMNBs at 37°C for 1 h. (a) The captured grE2 protein by IMbeads (Lane 1) and total used grE2 protein (Lane 2) were visualized by SDSP-PAGE and confirmed by western blot (Lane 3 and 4). (b) Detection of the binding to CSFV in IMNBs. 1000, 500, 400, 300, 200, and 100 <i>μ</i>l of CSFV (Lanes 1 to 6) propagated in ST cell culture were incubated with IMNBs and the size of 560 bp E2 gene was amplified by RT-PCR.</div>

BioMed Research International

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Figure 5

Figure 5: Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots 

Figure 5 | Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots 