[Retracted] α₁-Adrenergic Receptor Blockade by Prazosin Synergistically Stabilizes Rat Peritoneal Mast Cells

Figure 6

Effects of α₁- or α₂-adrenergic receptor antagonists on mast cell degranulation. (a) Effects of prazosin on mast cell degranulation and membrane capacitance. (A) After the mast cells were incubated in the external solutions containing no drug or different concentrations (0.01, 0.1, and 1 μM) of prazosin, exocytosis was induced by compound 48/80. The numbers of degranulating mast cells were expressed as percentages of the total mast cell numbers in selected bright fields. (B) After the mast cells were incubated in the external solutions containing no drug or different concentrations (0.01, 0.1, and 1 μM) of prazosin, the whole-cell recording configuration was established in single mast cells and dialysis with 100 μM GTP-γ-S was started. The GTP-γ-S-induced increase in the Cm (⊿Cm) was calculated. (b) Effects of yohimbine on mast cell degranulation. After the mast cells were incubated in the external solutions containing no drug or different concentrations (10 and 100 μM and 1 mM) of yohimbine, exocytosis was induced by compound 48/80. The numbers of degranulating mast cells were expressed as percentages of the total mast cell numbers in selected bright fields. ^# vs. incubation in the external solution alone. Values are . Differences were analyzed by ANOVA followed by Dunnett’s test.

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