Research Article

Silencing ARAF Suppresses the Malignant Phenotypes of Gallbladder Cancer Cells

Figure 2

Downregulated expression of ARAF inhibits GBC cell proliferation. (a, b) RT-qPCR and western blot analysis were performed to confirm the effect of ARAF silencing. (c) Cell proliferation assays of ARAF silencing GBC cells. (d) Detection of the protein levels of PCNA in ARAF siRNA and siRNA control groups. (e, f) Knockdown of ARAF inhibited colony formation of both GBC cell lines. Colonies were counted only if a single clone contained more than 50 cells. .