Molecular Mechanism of HSF1-Upregulated ALDH2 by PKC in Ameliorating Pressure Overload-Induced Heart Failure in Mice

Figure 2

The expression of HSF1, ALDH2, and ALDH2 upregulated or downregulated HSF1 transgene mice cardiac remodeling and heart function after TAC 4 weeks. (a) The relative mRNA level of the mouse heart tissue (sham: sham-operated mice; WT: wild-type mice; HSF1 TG: HSF1 transgene mice; HSF1 KO: HSF1 knockout mice). (b) The relative mRNA and protein levels of the mouse heart tissue (sham: sham-operated mice; WT: wild-type mice; HSF1 TG: HSF1 transgene mice; HSF1 KO: HSF1 knockout mice). (c) TAC HSF1 transgene mice heart histologic section: HE (1.25x) and Masson (5.0x) staining and echocardiography measure. (d) The relative protein expression of ALDH2 in the mouse heart tissue in each group. Ad-ALDH2-shRNA: mice are injected with adenovirus-expressing ALDH2 shRNA; Ad-control: mice are injected with blank adenovirus; Ad-ALDH2: mice are injected with adenovirus-expressing ALDH2; LVW/BW: left ventricular weight/body weight; LVID, d: left ventricular internal dimension, diastolic; LVEF: left ventricular ejection fraction. , vs. sham, ^#, ^## vs. WT TAC, .

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