Arctigenin Inhibits Glioblastoma Proliferation through the AKT/mTOR Pathway and Induces Autophagy

Figure 3

ARG regulated cell cycle and apoptosis of U87MG and T98G. (a) The cell cycle was determined by the PI/RNase staining buffer assay, and U87MG and T98G were, respectively, treated with different concentrations of ARG for 48 h, and then the cells were harvested. The cells were mixed with ethanol overnight, and the results were measured with an Accuri C6 flow cytometry after 15 min. (b) Apoptosis was detected by the PI-FITC-annexin assay and measured within 1 h, and U87MG and T98G were treated with different concentrations of ARG for 48 h, and the cells were analyzed by flow cytometry. The annexin V-FITC axis represented the cells that were early apoptotic, and the PI axis represented the cells in the mid-late stage of apoptosis. Data was expressed as the . vs. the control group. All experiments were repeated three times.

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