BioMed Research International / 2020 / Article / Fig 4

Research Article

Arctigenin Inhibits Glioblastoma Proliferation through the AKT/mTOR Pathway and Induces Autophagy

Figure 4

ARG induced autophagy. (a, b) U87MG and T98G were treated with different concentrations of ARG, and the whole cell lysate was subject to LC3B-II and P62 immunoblotting. β-Actin was used as a housekeeping protein. (c) U87MG and T98G were transfected with LC3B-GFP plasmids as the control, and the LC3-GFP puncta were observed under the fluorescence microscope. (d) Counting on the number of LC3B-GFP puncta per cell. Data was expressed as the . vs. the control group. All experiments were repeated three times.

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