Arctigenin Inhibits Glioblastoma Proliferation through the AKT/mTOR Pathway and Induces Autophagy

Figure 6

SC79 regulated glioma cell autophagy and apoptosis. (a, b) After 48 h of incubation with ARG and cotreated with SC79 (5 μg/μL), the cells were lysed and used for Western blotting. (c, d) To verify the protein expression levels of AKT, P-AKT, mTOR, and P-mTOR, Western blot was used to ensure the protein level of Beclin-1, LC3B, and P62. β-Actin was used as a housekeeping protein. (e) Quantification of the number of GFP-LC3 puncta per cell. Data was expressed as the . vs. the control group. All experiments were repeated three times.

(a)

(b)

(c)

(d)

(e)