Wubeizi Ointment Suppresses Keloid Formation through Modulation of the mTOR Pathway

Figure 3

The effect of Wubeizi ointment on the mRNA and protein levels in keloid tissues. (a) The inhibitory effect of Wubeizi ointment on the mRNA levels of PIK3CA, PTEN, Akt1, and mTOR in keloid tissues. After 30 days of Wubeizi ointment treatment, mice were sacrificed, and the keloid tissues were disassociated. The mRNA levels of PIK3CA, PTEN, Akt1, and mTOR in keloid tissues were measured by qPCR. The grey values were quantified with the gel imaging analysis system. The RQ of mRNA was calculated and presented as . , . (b) The inhibitory effect of Wubeizi ointment on the protein levels of p-PI3K, PTEN, p-Akt, and p-mTOR in keloid tissues. After 30 days of Wubeizi ointment treatment, mice were sacrificed, and the keloid tissues were disassociated. The protein levels of p-PI3K, PTEN, p-Akt, and p-mTOR in keloid tissues were determined by western blot. Representative pictures are shown. Band intensities were quantified with the Image Pro-Plus image analysis system. The relative ratios of proteins to actin band density were presented as . , .

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