HYAL1 Is Downregulated in Idiopathic Pulmonary Fibrosis and Inhibits HFL-1 Fibroblast Proliferation When Upregulated

Figure 8

Western blot analysis of TGFβR2, BMPR2, and SMAD1/5/9 in negative controls and HYAL1-overexpressing HFL-1 fibroblasts. (a) Western blot of TGFβR2, BMPR2, and SMAD1/5/9 in negative controls () and HYAL1-overexpressing HFL-1 fibroblasts (); NC: HFL-1 fibroblasts infected with lentivirus containing CON335 empty vector (3 independent replicates); OE: HFL-1 fibroblasts infected with lentivirus containing HYAL1 recombinant plasmid (3 independent replicates). (b) Relative protein levels of TGFβR2, BMPR2, and SMAD1/5/9 in negative controls () and HYAL1-overexpressing HFL-1 fibroblasts (); NC: HFL-1 fibroblasts infected with lentivirus containing CON335 empty vector (3 independent replicates); OE: HFL-1 fibroblasts infected with lentivirus containing HYAL1 recombinant plasmid (3 independent replicates). All values were calculated using the unpaired test. .

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