Statistical Experimental Design Optimization of Microbial Proteases Production under Co-Culture Conditions for Chitin Recovery from Speckled Shrimp Metapenaeus monoceros By-Product

Table 1

Effect of increased shrimp by-product concentration on bacterial growth and protease production by Anoxybacillus kamchatkensis M1V.

Concentration of shrimp by-product (g/L)	Protease activity (U/mL)	Biomass (g/L)	Yield (IU protease/g of added by-product)
10	1,200	1.8	120,000
15	1,600	3	106,666
20	1,850	6.07	92,500
25	2,000	7	80,000
30	1,400	5	466,66
35	1,150	5.04	32,857
40	1,000	3	25,000