Statistical Experimental Design Optimization of Microbial Proteases Production under Co-Culture Conditions for Chitin Recovery from Speckled Shrimp Metapenaeus monoceros By-Product

Table 6

Screening of factors by the Box–Behnken design.

Code	Temperature (°C)	Inoculum size of strain VP3 (A600 nm)	Inoculum size of strain M1V (A600 nm)	Volume (mL)	Protease activity (U/mL)
1	−1	−1	0	0	5,532
2	−1	1	0	0	4,740
3	1	−1	0	0	7,114
4	1	1	0	0	7,637
5	0	0	−1	−1	9,437
6	0	0	−1	1	6,645
7	0	0	1	−1	7,364
8	0	0	1	1	4,400
9	−1	0	0	−1	5,038
10	−1	0	0	1	1,628
11	1	0	0	−1	6,400
12	1	0	0	1	4,503
13	0	−1	−1	0	9,764
14	0	−1	1	0	9,000
15	0	1	−1	0	11,310
16	0	1	1	0	5,164
17	−1	0	−1	0	6,168
18	−1	0	1	0	3,864
19	1	0	−1	0	6,650
20	1	0	1	0	6,182
21	0	1	0	−1	5,052
22	0	1	0	1	5,134
23	0	1	0	−1	4,322
24	0	1	0	1	5,882
25	0	0	0	0	5,140
26	0	0	0	0	6,472
27	0	0	0	0	6,491