PCK1 Deficiency Shortens the Replicative Lifespan of Saccharomyces cerevisiae through Upregulation of PFK1

Figure 1

PCK1 deletion shortened the lifespan of S. cerevisiae. (a) Real-time qPCR was used to detect the PCK1 expression levels in BY4742 and pck1Δ strain. Columns, mean of at least three independent experiments; bars, SEM. , comparison between two groups as indicated. (b) RLS curves of BY4742 and pck1Δ strains. The survival curves were generated from lifespan data, and BY4742 was considered as the control. Numbers in parentheses are the median RLS and cell number. (c) Real-time qPCR was utilized to monitor the PCK1 expression levels in BY4742 and PCK1 OE strain. Columns, mean of at least three independent experiments; bars, SEM. , comparison between two groups as indicated. (d) RLS curves of BY4742 and PCK1 OE strains. The survival curves were generated from lifespan data, and BY4742 was considered as the control. Numbers in parentheses are the median RLS and cell number. (e) The growth curves of BY4742 and pck1Δ yeast cells based on monitoring (A600) at indicated times with or without glucose. (f) BY4742 and pck1Δ yeast cells were diluted (1 : 10) sequentially and cultured on YPD plates with or without glucose. (g) BY4742 and pck1Δ yeast cells were cultured in YPD medium, in all cases, yeast was inoculated at ∼10⁴ CFU/mL. (h) The growth curves of BY4742 and PCK1 OE yeast cells based on monitoring (A600) at indicated times with or without glucose. (i) BY4742 and PCK1 OE yeast cells were diluted (1 : 10) sequentially and cultured on YPD plates with or without glucose. (j) BY4742 and PCK1 OE yeast cells were cultured in YPD medium; in all cases, yeast was inoculated at ∼10⁴ CFU/mL.

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