PCK1 Deficiency Shortens the Replicative Lifespan of Saccharomyces cerevisiae through Upregulation of PFK1

Figure 3

Identification of differentially expressed mRNAs in PCK1 deletion strains. (a) mRNA expression profiles of BY4742 and pck1Δ strain (n = 2) were evaluated by deep sequencing. The heat map was generated from the hierarchical cluster analysis to show a distinguishable mRNA expression profile between BY4742 and pck1Δ strain. The color is determined by the ratio between the mRNA signal value of BY4742 and pck1Δ strain. (b) Volcano map of differentially expressed genes. Each dot in the figure represents a specific gene, with the purple dots indicating significantly upregulated genes, the green dots indicating significantly downregulated genes, and the black dots indicating nonsignificant genes. (c) Differentially expressed gene KEGG pathway enrichment scatter plot. Each dot in the figure is a KEGG pathway, and the ordinate text indicates the pathway name of KEGG, and the classification description is as shown in the right class legend. The abscissa is expressed as the enrichment rate, and the formula is as follows: Enrich_factor = GeneRatio/BgRatio; the color indicates the significance of the enrichment. (d) The mRNA expression levels of GPM1, PGK1, TDH2, PGI1, FBA1, ENO1, PFK1, and HXK2 were examined in BY4742 and pck1Δ strains. (e) The mRNA expression levels of GPM1, PGK1, TDH2, PGI1, FBA1, ENO1, PFK1, and HXK2 were examined in BY4742 and PCK1 OE strains. Columns, mean of at least three independent experiments; bars, SEM. , , comparison between two groups as indicated.

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