Probucol Prevents Diabetes-Induced Retinal Neuronal Degeneration through Upregulating Nrf2

Figure 3

Ocular histological and oxidative injury analysis examination of the effects of probucol on streptozotocin- (STZ-) induced diabetic retinopathy in C57BL/6 mice. (a) Representative images of H&E staining showing the morphology of the eyes from the control group, DM group, DM + DMSO group, and DM + PB group at week 12. (b) Representative images of Dihydroethidium (DHE) staining in retinas in each group. (c) Representative images of TUNEL staining in retinas in each group.(d-e) Nuclei number counting of the inner nuclear layer and outer nuclear layer demonstrated that there are less nuclei in the DM and DM + DMSO groups. (f) Bar graphs representing quantification of tissues stained with DHE. (g-h) Bar graphs representing quantification of tissues stained with TUNEL demonstrated that there are more TUNEL-positive cells in the DM and DM + DMSO groups. Mean ± SEM, N = 4 mice per group. ; ; compared with the control group; , ; compared with the DM group. Scale bar: 50 μm.

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