A Simple Method for the Design and Development of Flavivirus NS1 Recombinant Proteins Using an In Silico Approach

Table 2

MHC class I binding peptide common in DENV types predicted by the T-cell epitope prediction tool at IEDB.

Start no. of amino acid position	Major allele	Peptide of DENV-1	Peptide of DENV-2	Peptide of DENV-3	Peptide of DENV-4
20	HLA-B53:01	FVTNEVHTW	FITDNVHTW	FVTNEVHTW	FVVDNVHTW
26	HLA-A32:01	HTWTEQYKF	HTWTEQYKF	HTWTEQYKF	HTWTEQYKF
38	HLA-B07:02	SPKRLSAAI	SPSKLASAI	SPKRLATAI	SPARLASAI
107	HLA-B53:01	QPMEHKYSW	QPTELKYSW	QPMELKYSW	PASDLKYSW
160	HLA-B58:01	FGIFTTNIW	FGVFTTNIW	FGVFTTNIW	FGMFTTNIW
243	HLA-B07:02	IPKIYGGPI	IPKNFAGPV	IPKSLAGPI	IPKSYAGPF
252	HLA-B15:01	SQHNYRPGY	SQHNYRPGY	SQHNHRPGY	SQHNYRQGY
303	HLA-B58:01	VTGKIIHEW	ASGKLITEW	VSGKLIHEW	ASGKLVTQW

These data were extracted by filtering at below 0.6 percentile rank. Bold letters indicate that the peptide is shared with the MHC class II binding core peptide.