Beneficial Effects of Crocin against Depression via Pituitary Adenylate Cyclase-Activating Polypeptide

Figure 5

PACAP was involved in the neuroprotective effects of crocin in the CORT model of PC12 cells. (a) Knockdown of PACAP with PACAP siRNA. The cells were observed under the fluorescence microscope. (b-c) Western blotting analysis of the expression of PACAP after transfection with PACAP siRNA. -test, , . (d) Hoechst33258 staining and observation of PC12 cells with fluorescence microscope after transfection with PACAP siRNA and crocin. (e) Effects of crocin in cell viability of PC12 cell after transfection with PACAP siRNA. ANOVA, , . and versus control group; and versus CORT group, and versus CORT+crocin group.

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