Research Article
Uniaxial Static Strain Promotes Osteoblast Proliferation and Bone Matrix Formation in Distraction Osteogenesis In Vitro
Table 1
Forward (F) and reverse (R) primers used for quantitative RT-PCR.
| | Gene | GenBank no. | Primer sequence |
| | GAPDH | XR_009170.1 | F: TATGACTCTACCCACGGCAAGT | | R: ATACTCAGCACCAGCATCACC | | PCNA | NM_022381.3 | F: GGGTGAAGTTTTCTGCGAGTG | | R: GACAGTGGAGTGGCTTTTGTGA | | ALP | NM_013059.1 | F: GACCCTGCCTTACCAACTCATT | | R: GTGGAGACGCCCATACCATCT | | Runx2 | NM_053470.2 | F: CTTCGTCAGCGTCCTATCAGTTC | | R: CAGCGTCAACACCATCATTCTG | | OCN | X04141.1 | F: CCTCTCTCTGCTCACTCTGCTG | | R: ACCTTACTGCCCTCCTGCTTG | | Collagen type I | BC133728.1 | F: TCTGACTGGAAGAGCGGAGAG | | R: GAGTGGGGAACACACAGGTCT | | HIF-1α | NM_024359.1 | F: GCATCTCCACCTTCTACCCAAGT | | R: ACTCTCTTTCCTGCTCTGTCTGG | | VEGF | AY702972.1 | F: CTGTACCTCCACCATGCCAAGT | | R: AGATGTCCACCAGGGTCTCAAT |
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