TRIM32 Promotes the Growth of Gastric Cancer Cells through Enhancing AKT Activity and Glucose Transportation

Figure 4

Knockdown of TRIM32 suppressed GC cells growth. (a) and (b) stand for cell proliferation that was detected 0, 24, 48, and 72 hours after transfection with siNC, siTRIM32-1, and siTRIM32-2 in NCI-N87 and MKN74 cells, respectively. vs. siNC, vs. siNC. (c) The apoptosis profile of siNC, siTRIM32-1, and siTRIM32-2 transfected into NCI-N87 and MKN74 cells, respectively. vs. siNC. (d) Glucose transport activity measured using the fluorescent glucose analog 2-NBDG in NCI-N87 and MKN74 cells transfected with siNC, siTRIM32-1, and siTRIM32-2, respectively. vs. siNC. (e) The production of lactate in NCI-N87 and MKN74 cells transfected with siNC, siTRIM32-1, and siTRIM32-2, respectively. vs. siNC. (f) and (g) stand for the protein level of AKT, p-AKT, GLUT1, and HKII in NCI-N87 and MKN74 cells transfected with siNC, siTRIM32-1, and siTRIM32-2, respectively. vs. siNC.

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