TRIM32 Promotes the Growth of Gastric Cancer Cells through Enhancing AKT Activity and Glucose Transportation

Figure 5

The function of TRIM32 was suppressed by the P13/AKT inhibitor LY294002. (a) Cell proliferation was detected 0, 24, 48, and 72 hours after transfection with oeNC, oeTRIM32, oeNC + LY294002, and oeTRIM32 + LY294002 in MKN45 cells, respectively. vs. oeNC, vs. oeNC. (b) Cell proliferation was detected 0, 24, 48 and 72 hours after transfection with oeNC, oeTRIM32, oeNC + 2-DG, and oeTRIM32 + 2-DG in MKN45 cells, respectively. vs. oeNC, vs. oeNC, vs. oeNC. (c) The apoptosis profile of oeNC, oeTRIM32, oeNC + LY294002, and oeTRIM32 + LY294002 in MKN45 cells, respectively. vs. oeNC, vs. oeNC. (d) Glucose transport activity measured using the fluorescent glucose analog 2-NBDG in MKN45 cells transfected with oeNC, oeTRIM32, oeNC + LY294002, and oeTRIM32 + LY294002. vs. oeNC. (e) The production of lactate in MKN45 cells transfected with oeNC, oeTRIM32, oeNC + LY294002, and oeTRIM32 + LY294002. vs. oeNC. vs. oeNC. (f). The protein level of AKT, p-AKT, GLUT1, and HKII in MKN45 cells transfected with oeNC, oeTRIM32, oeNC + LY294002, and oeTRIM32 + LY294002. vs. oeNC.

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