FOXA1 Expression in Nasopharyngeal Carcinoma: Association with Clinicopathological Characteristics and EMT Markers

Table 2

Primer sequences for RT-PCR and RT-qPCR.

Gene		Primer sequences (5-3)	Product size (bp)	Annealing T (°C)
FOXA1	Forward	CGCTTCGCACAGGGCTGGAT	143	62
	Reverse	TGCTGACCGGGACGGAGG AG
E-cadherin	Forward	CGGGAATGCAGTTGAGGATC	199	60
	Reverse	AGGTGGTGTAAGCGATGGC
N-cadherin	Forward	ATTGGACCATCACTCGGCTTA	158	58
	Reverse	CACACTGGCAAACCTTCACG
LMP1	Forward	AGCCCTCCTTGTCCTCTATTCCTT	253	60
	Reverse	ACCAAGTCGCCAGAGAATCTCC
TWIST	Forward	CAAGCTGCAGCTATGTGGC	168	57
	Reverse	TGTCCATTTTCTCCTTCTCTGG
Vimentin	Forward	CCCTCACCTGTGAAGTGGAT	271	60
	Reverse	TCCAGCAGCTTCCTGTAGGT
Snail	Forward	GATGCCGCGCTCCTTCC	265	61
	Reverse	GGGGACTCACTCGCCCC
GAPDH	Forward	GCTCTCTGCTCCTCCTGTTC	122	60
	Reverse	CGCCCAATACGACCAAATCC