Dysregulated Bone Metabolism Is Related to High Expression of miR-151a-3p in Severe Adolescent Idiopathic Scoliosis

Figure 4

Validation of miR-151a-3p targeting GREM1 mRNA. (a) Schematic figure demonstrating alignment of miR-151a-3p, wild-type, and mutant GREM1 3UTR sequences. (b) Luciferase reporter assay was performed after HEK-293T cells were cotransfected with a random sequence. miR-151a-3p or control mimic as well as the plasmid vector with wild-type or mutant GREM1 3UTR. versus the control group ().

(a)

(b)