Dysregulated Bone Metabolism Is Related to High Expression of miR-151a-3p in Severe Adolescent Idiopathic Scoliosis

Figure 5

GREM1 expression was inhibited by miR-151a-3p in primary osteoblasts. (a) Primary osteoblasts extracted from severe AIS samples were incubated with or without BMP2 and transfected with miR-151a-3p mimic or inhibitor. GREM1 mRNA expression levels were determined by quantitative real-time PCR. The mean value of the untreated group was set to 1.0. between the indicated two groups (). (b) Mineralization of primary osteoblasts incubated with BMP2 and transfected with miR-151a-3p mimic for two weeks was detected and quantified by Alizarin Red S staining. (c) Quantification of mineralization was performed. between the indicated two groups ().

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