Pathotyping and Antibiotic Resistance Profiling of Escherichia coli Isolates from Children with Acute Diarrhea in Amatole District Municipality of Eastern Cape, South Africa

Table 1

Primer sequences used for the molecular confirmation of E. coli isolates and identification of its pathotypes.

Target strain/gene	Primer sequence 5-3	Annealing temp (°C)	Band size (bp)	References
E. coli (uidA)	AAAACGGCAAGAAAAAGCAG ACGCGTGGTTACAGTCTTGCG; TCA ATG CAG TTC CGT TAT CAG TT	50; 55	147; 450	[20]; [21]
eaeA (EHEC)	GTA AAG TCC GTT ACC CCA ACC TG; GCACACGGAGCTCCTCAGTCTCC	56	218	[21]
lt (ETEC)	TTCATCCTTTCAATGGCTTT; AGCTCAGGCAATGAAACTTTGAC	54	618	[22]
vir (EIEC)	TGGGCTTGATATTCCGATAAGTC; TCAATGCAGTTCCGTTATCAGTT	55	482	[21]
eae (EPEC)	GTAAAGTCCGTTACCCCAACCTG; CACAGGCAACTGAAATAAGTCTGG	56	378	[22]
aafII (EAEC)	ATTCCCATGATGTCAAGCACTTC; GAACGTTGGTTAATGTGGGGTAA	56	542	[22]
daaE (DAEC)	TATTCACCGGTCGGTTATCAGT