High Glucose Induces the Loss of Retinal Pericytes Partly via NLRP3-Caspase-1-GSDMD-Mediated Pyroptosis

<div>Overexpression of GSDMD-N facilitated high glucose-induced inflammation and pyroptosis. Plasmids expressing GSDMD-N with an N-terminal GFP tag, and pEGFP-C1 control plasmid that express GFP but cannot overexpress GSDMD-N, were constructed to determine the effect of overexpressed GFP-GSDMD-N fusion protein in HRPs under 30 mM high glucose (HG) stimulation and DEVD or YVAD treatment by western blotting or fluorescence images (×400). High glucose-induced (b) IL-1<i>β</i> and IL-18 release from <i>HRPs</i>, (c) pore formation, and (d) LDH release were significantly facilitated by the overexpression of GSDMD-N. 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BioMed Research International

fig3

Figure 3

Figure 3: High Glucose Induces the Loss of Retinal Pericytes Partly via NLRP3-Caspase-1-GSDMD-Mediated Pyroptosis 