The Establishment of Esophageal Precancerous Lesion Model by Using p53 Conditional Knockout Mouse in Esophageal Epithelium

Figure 1

Breeding strategy and genotype identification. (a) Flow chart of breeding design for the mice with desired genotypes of p53^flox/floxCre^−/− and p53^flox/floxCre^+/− by using the mice with genotypes of B6.p53^flox/flox and ED-L2-Cre^+/−. (b) The genotype of p53^flox/flox mice. (c) The genotype of F1 generation mice. (d) The genotype of F2 generation mice. M: marker; PC: positive control; WT: wild type control; NTC: no temple control.

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