The Establishment of Esophageal Precancerous Lesion Model by Using p53 Conditional Knockout Mouse in Esophageal Epithelium

Figure 2

The expression of p53 in the esophageal mucosa of p53 KO mice was significantly reduced. (a) The p53 mRNA levels in esophageal mucosa, esophageal muscle layer, tongue, and stomach tissues of the two genotype mice (KO mice and Loxp mice) were detected by qPCR. Three mice were taken from each group, and each mouse had three replicates. ES S: esophageal mucosa; ES M: esophageal muscle layer ( means , means ). (b) The expression of p53 protein was detected by immunohistochemistry analysis. P53 expressions stain a brown color and the nuclei are stained blue with hematoxylin. The staining of p53 was analyzed using HistoQuest 4.0 software. Arrows refer to the esophageal mucosa (n = 3, means ). (c) HE staining of esophageal tissues in 20-week-old p53 KO mice and Loxp mice. Scale bar: 100 μm.

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