[Retracted] Circular RNA circCCDC66 Contributes to Malignant Phenotype of Osteosarcoma by Sponging miR-338-3p to Upregulate the Expression of PTP1B

Figure 4

circCCDC66 acted as a sponge for miR-338-3p. (a) circCCDC66 was more distributed in the cytoplasm. (b, c) The relative expression levels of 4 miRNAs in the circCCDC66 probe group and the control probe group in SW1353 and U2OS cells, respectively. (d) Predicted binding sites for miR-338-3p with the circCCDC66 and the design of wild-type (WT) and mutant (MUT) circCCDC66 luciferase reporter vector constructs. (e) Effect of miR-338-3p mimics on circCCDC66 WT and circCCDC66 MUT was detected through dual-luciferase reporter assay. (f) The expression levels of miR-338-3p in SW1353 and U2OS cells transfected with the si-circCCDC66 or si-NC was detected by qRT-qPCR assay. .

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