Icariin Ameliorates Lower Back Pain in Rats via Suppressing the Secretion of Cytokine-Induced Neutrophil Chemoatractant-1

Figure 5

ICA attenuated the LBP by suppressing the expression of CINC-1. (a) A comparison between ICA and reparixin (the inhibitor of the CINC-1 receptor) suggested that inhibition of the production of CINC-1 was the key mechanism to attenuate discogenic LBP. (b) Percutaneous injection of exogenous CINC-1 aggravated the LBP even on treatment with ICA (<0.05, <0.01, and <0.001 when compared between the puncture+saline group and the puncture+ICA 100 mg/kg group. ⁺<0.05, ⁺⁺<0.01, and ⁺⁺⁺<0.001 when compared between the puncture+saline group and the puncture+reparixin group. ^&<0.05, ^&&<0.01, and ^&&&<0.001 when compared between the puncture+ICA 100 mg/kg group and the puncture+reparixin group. ^×××<0.001 when compared between the puncture+saline group and the puncture+ICA 100 mg/kg+CINC-1 group. ^▲▲▲<0.001 represented the comparison between the puncture+ICA 100 mg/kg group and the puncture+ICA 100 mg/kg+CINC-1 group. The data are shown as . for each group. One-way or two-way ANOVA and Tukey’s multiple comparison test were used for statistical analysis).

(a)

(b)