Histone Deacetylase Inhibition Attenuates Aortic Remodeling in Rats under Pressure Overload

Figure 3

Effect of MGCD on aortic remodeling in rats after TAC. Histological analysis of the aortic wall from the sham (), sham plus MGCD (), TAC (), and TAC plus MGCD groups () was performed. (a) For hematoxylin and eosin staining (H&E), the thickness of the aortic wall, both in the aortic adventitia and media, was higher in the TAC rats than in the sham rats; the thickness was attenuated by MGCD treatment in both rats. (b) For trichrome staining, fibrosis of the aortic wall, especially in the adventitia (stained blue), was higher in the TAC rats than in the sham rats; the fibrosis was attenuated by MGCD treatment in both rats. (c) For Verhoeff-Van Gieson (VVG) staining, the elastin fibers on the aortic wall, especially in the adventitia (stained dark purple), was higher in the TAC rats than in the sham rats; MGCD treatment attenuated elastin content in the TAC plus MGCD rats. Scale bars are 50 μm.