Differential Expression and Bioinformatic Analysis of the circRNA Expression in Migraine Patients

Figure 1

Identification of migraine-associated circRNA expression profiles and qRT-PCR confirmation of differential circRNA expression profiles. Differentially expressed circRNAs are shown in a volcano plot (a) and a scatter plot (b). Those circRNAs that were up- and downregulated by at least twofold in migraine patients relative to controls are shown in red, respectively (). Gray dots correspond to circRNAs for which no differential expression was observed. (c) Hierarchical clustering of these circRNAs. CircRNAs that were differentially expressed in the plasma of migraine patients relative to healthy controls are shown with corresponding chromosomal locations. While circRNAs were identified corresponding to all chromosomes, many were concentrated on chromosomes 1 and 2 (d). CircRNA types are shown, with may being derived from protein-coding exons, some being derived from introns, and a few from intergenic regions (e).The relative expression of the 8 indicated circRNAs in migraine and control patient samples, as assessed via microarray (f) and qRT-PCR (g). The expression was normalized to control samples. samples per group. Data are means with SD. , Student’s -test.

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