Prevalence and Genotype Distribution of Giardia duodenalis in Rabbits in Shandong Province, Eastern China

Table 1

Nest PCR primers and annealing temperatures used in this study.

Gene	Primer	Sequence (5′-3′)	Annealing temperature (°C)	Length (bp)	Reference
bg	F1	AAGCCCGACGACCTCACCCGCAGTGC	55		[2]
	R1	GAGGCCGCCCTGGATCTTCGAGACGAC
	F2	GAACGAACGAGATCGAGGTCCG	55	511
	R2	CTCGACGAGCTTCGTGTT
gdh	F1	TTCCGTRTYCAGTACAACTC	50		[2]
	R1	ACCTCGTTCTGRGTGGCGCA
	F2	ATGACYGAGCTYCAGAGGCACGT	65	530
	R2	GTGGCGCARGGCATGATGCA
tpi	F1	AAATIATGCCTGCTCGTCG	55		[2]
	R1	CAAACCTTITCCGCAAACC
	F2	CCCTTCATCGGIGGTAACTT	55	530
	R2	GTGGCCACCACICCCGTGCC