Research Article
An UPLC-MS/MS Method for Simultaneous Quantification of the Components of Shenyanyihao Oral Solution in Rat Plasma
Table 6
Stability of the components in rat plasma sample.
| Compounds | Concentration (ng/ml) | Room temperature (3 h, 25°C) | Autosampler (24 h, 4°C) | Three freeze/thaw cycles | Long term (30 day, −80°C) | Precision (RSD%) | Accuracy (RE%) | Precision (RSD%) | Accuracy (RE%) | Precision (RSD%) | Accuracy (RE%) | Precision (RSD%) | Accuracy (RE%) |
| Stachydrine | 10.00 | 4.62 | -9.23 | 2.66 | -9.94 | 4.44 | -7.37 | 4.69 | -5.94 | 250.00 | 0.81 | 0.09 | 4.06 | -6.0 | 2.59 | 6.17 | 3.49 | 2.03 | 2000.00 | 4.49 | 3.14 | 3.82 | 3.07 | 3.55 | 5.27 | 3.37 | 4.64 | Danshensu | 2.00 | 5.29 | 7.09 | 5.29 | 7.09 | 6.92 | -2.70 | 8.08 | 6.79 | 50.00 | 10.89 | -10.20 | 10.89 | -10.20 | 1.58 | -12.52 | 5.55 | -4.84 | 400.00 | 2.78 | 4.73 | 2.78 | 4.73 | 0.82 | 0.91 | 1.22 | 2.60 | Chlorogenic acid | 0.20 | 6.13 | -3.40 | 1.98 | -1.57 | 5.29 | -3.89 | 4.57 | -0.48 | 5.00 | 0.45 | 11.70 | 0.80 | 13.89 | 4.07 | 9.84 | 1.25 | 10.93 | 40.00 | 0.66 | 11.51 | 0.65 | 14.17 | 1.20 | 13.15 | 0.53 | 12.59 | Protocatechuic acid | 0.20 | 3.69 | 7.78 | 1.00 | 12.84 | 1.60 | 1.80 | 3.87 | 5.18 | 5.00 | 2.55 | 10.39 | 1.99 | 5.52 | 1.03 | 8.49 | 3.58 | 8.11 | 40.00 | 0.95 | 10.48 | 1.97 | 8.98 | 2.29 | 8.55 | 0.50 | 7.28 | Plantamajoside | 0.10 | 2.00 | 9.23 | 5.21 | 7.94 | 2.91 | 6.68 | 7.05 | 4.14 | 2.50 | 1.28 | -90.31 | 5.08 | -91.35 | 2.59 | -91.11 | 3.79 | -90.84 | 20.00 | 1.36 | 5.03 | 1.94 | -0.13% | 2.87 | -5.26 | 1.20 | -0.43 | Aesculetin | 0.20 | 5.16 | -1.16 | 1.51 | 4.88 | 3.02 | 2.69 | 4.20 | -0.62 | 5.00 | 2.41 | 7.25 | 0.89 | 8.31 | 1.95 | 9.94 | 1.79 | 6.76 | 40.00 | 2.07 | 11.25 | 4.89 | 9.87 | 1.90 | 7.39 | 3.61 | 7.41 | Isoquercitrin | 0.20 | 2.65 | 3.57 | 2.55 | 4.81 | 2.56 | 8.27 | 1.88 | 0.43 | 5.00 | 0.66 | 5.18 | 0.48 | 5.40 | 1.58 | 4.20 | 0.51 | 5.56 | 40.00 | 0.54 | 11.02 | 2.16 | 11.09 | 2.43 | 10.30 | 0.63 | 8.27 | Ferulic acid | 2.00 | 3.09 | 5.81% | 11.12 | 6.79 | 7.50 | 4.93 | 4.72 | 7.22 | 50.00 | 2.37 | 4.96 | 0.67 | 4.55 | 1.47 | 6.41 | 0.99 | 5.32 | 400.00 | 0.38 | 3.57 | 2.20 | 4.12 | 1.39 | 4.29 | 0.06 | 2.22 | Baicalin | 10.00 | 1.38 | -6.39 | 4.18 | -5.56 | 5.63 | -0.46 | 4.01 | -6.58 | 250.00 | 0.37 | -10.18 | 1.38 | -11.90 | 2.72 | -0.02 | 4.76 | -5.18 | 2000.00 | 7.12 | -3.62 | 4.10 | -6.54 | 5.25 | -7.42 | 3.31 | -5.38 | Baicalein | 2.00 | 6.37 | -3.82 | 2.29 | -7.65 | 1.69 | -3.44 | 2.30 | -10.32 | 50.00 | 2.29 | -9.87 | 2.99 | -8.08 | 3.72 | -1.39 | 4.60 | -5.23 | 400.00 | 5.15 | 0.51 | 4.11 | -5.84 | 3.38 | -0.69 | 3.42 | -8.34 |
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