Engineered IL-7 Receptor Enhances the Therapeutic Effect of AXL-CAR-T Cells on Triple-Negative Breast Cancer

Figure 2

The effect of C7R on the activation of AXL-CAR-T cells. (a) Determination of the optimal target ratio. A total of 1 × 10⁴ target cells were incubated with T cells at different ratios. The concentrations of LDH released in the supernatant were measured 24 hours later. (b) A total of 1 × 10⁴ target cells were incubated with T cells for 24 h at a ratio of 2 : 1. The expression level of CD69 in T cells was measured by flow cytometry. (c) A total of 1 × 10⁴ target cells were incubated with T cells for 24 h at a ratio of 2 : 1, levels of IL-2 secretion were measured (n = 3, , ). (d) The concentrations of secreted IFNγ (n = 3, , ).

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