Engineered IL-7 Receptor Enhances the Therapeutic Effect of AXL-CAR-T Cells on Triple-Negative Breast Cancer

Figure 3

The effect of C7R on the proliferation and cytotoxicity of AXL-CAR-T cells. (a) A total of 1 × 10⁵ Mitomycin C-treated tumor cells were incubated with 2 × 10⁵ T cells. The Mitomycin C-treated tumor cells were refreshed every week. After three antigen stimulations, the proliferation of two sets of CAR-T cells was compared. Prior to the third stimulation, the two sets of CAR-T cells were prestained with Cell Trace Violet, and stained CAR-T cells were cultured with MDA-MB-231 at a ratio of 2 : 1 for 96 hours. The intensity of Cell Trace Violet in each group was measured by flow cytometry. (b) After two antigen stimulations, the ratio of CD8+ to CD4+ T cells was measured on day 18 (n = 3, ns, , ). (c) A total of 1 × 10⁴ tumor cells were incubated with T cells at a ratio of 2 : 1, and levels of LDH in the supernatant were measured 24 hours later to evaluate the cytotoxicity of T cells (n = 3, ).

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