Engineered IL-7 Receptor Enhances the Therapeutic Effect of AXL-CAR-T Cells on Triple-Negative Breast Cancer

Figure 5

In vivo cytokine release and the proliferation of T cells in mice. (a)–(e) The concentrations of interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-6 (IL-6), tumor necrosis factor-α (TNFα), and interferon-γ (IFNγ) in peripheral blood of mice, which was collected at 24 hours after the administration of T cells (n = 4, , , ). Measurements were performed with 100 μL peripheral blood using ELISA. (b) On day 27, 100 μL of peripheral blood was sampled from each mouse and the number of circulating human CD4+ and CD8+ T cells was determined by flow cytometry (n = 4, , ).

(a)

(b)

(c)

(d)

(e)

(f)