Apelin Promotes ECM Synthesis by Enhancing Autophagy Flux via TFEB in Human Degenerative NP Cells under Oxidative Stress

Table 1

Primer sequence for target gene.

Gene	Primer sequence
β-Actin	Forward: 5′-TCACCATGGATGATGATATCGC-3′
	Reverse: 5′-CGTGCTCGATGGGGTACTTCA-3
Apelin	Forward: 5′-CTGCTCTGGCTCTCCTTGAC-3′
	Reverse: 5′-ATGGGTCCCTTATGGGAGAG-3′
Collagen II	Forward: 5′-TTCAGCTATGGAGATGACAATC-3′
	Reverse: 5′-AGAGTCCTAGAGTGACTGAG-3′
Aggrecan	Forward: 5′-ATGCCCAAGACTACCAGTGG-3′
	Reverse: 5′-TCCTGGAAGCTCTTCTCAGT-3′
TFEB	Forward: 5′-GGAGGCGTGATGGTGAACTC-3
	Reverse: 5′-CTCACAACAGCCTGTGAGGT-3′
LC3	Forward: 5′-GTGAGTGTGTCCACGCCCAT-3′
	Reverse: 5′-AGGTTTCCTGGGAGGCGTAG-3′
P62	Forward: 5′-TGCCCCTCTTCTGTCTCATAGT-3′
	Reverse: 5′-CACTTGTTTTGCTGCCCTAAAT-3′